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S9396

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:
9031-54-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.1.4.12 (BRENDA, IUBMB)
MDL number:
MFCD00132362
Specific activity:
≥100 units/mg protein (Lowry)
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Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

≥100 units/mg protein (Lowry)

mol wt

34.2 kDa

foreign activity

Phospholipase C ≤1%

storage temp.

2-8°C

Gene Information

Bacillus cereus ATCC 10987 ... spH(2751530)

General description

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
  • in lipoprotein modification for the generation of cholesterol crystals in vitro
  • to aggregate LDL (low-density lipoprotein)

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. [1] Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems. [2]

Biochem/physiol Actions

Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

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Sphingomyelinase from Bacillus cereus buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Sigma-Aldrich

S9396

Sphingomyelinase from Bacillus cereus

Sphingomyelinase from Staphylococcus aureus buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

Sigma-Aldrich

S8633

Sphingomyelinase from Staphylococcus aureus

Sphingomyelinase from Bacillus cereus lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

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Sphingomyelinase, Staphylococcus aureus

Sigma-Aldrich

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Sphingomyelinase, Staphylococcus aureus

Gene Information

Bacillus cereus ATCC 10987 ... spH(2751530)

Gene Information

-

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

Gene Information

-

specific activity

≥100 units/mg protein (Lowry)

specific activity

100-300 units/mg protein (Lowry)

specific activity

≥100 units/mg protein

specific activity

≥100 units/mg protein

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

34.2 kDa

mol wt

-

mol wt

36873 Da

mol wt

-

foreign activity

Phospholipase C ≤1%

foreign activity

-

foreign activity

-

foreign activity

-

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000464513
0000464513
0000464512
0000464512
0000464511

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Global Trade Item Number

SKUGTIN
S9396-50UN04061832759760
S9396-25UN04061836961404

Questions

1–2 of 2 Questions  

  1. what is the concentration and volumn of this product s9396-25units

    1 answer

    1. The concentration and volume of this product vary from lot to lot. The protein content and activity of this enzyme are reported on the lot specific Certificate of Analysis. The volume may be calculated using this information. For example, a given lot that reports a protein content of 1 mg/mL and an activity of 300 units per milligram protein, will have a volume of 167 uL. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/US/en/product/sigma/s9396#product-documentation

      Please see the following link to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/230/s9396dat.pdf

      Helpful?

  2. Can you tell me concentration according to volume?????? Cat No: S9396-50UN

    1 answer

    1. Prot. Content (mg Protein/ml) and Enzym. Assay (Activity U/ml) are reported in batch specific Certificate of Analysis . Please see the link below to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/s9396#product-documentation

      Helpful?

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