Asp-N Protease

Name: Asp-N Protease
Brand: SIGMA

Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain

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About This Item

CAS Number:

9001-92-7

EC Number:

3.4.24.33 (BRENDA, IUBMB)

UNSPSC Code:

12352204

NACRES:

NA.56

EC Number:

232-642-4

MDL number:

MFCD02253021

eCl@ss:

32160410

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Properties

Product Name

Endoproteinase Asp-N from Pseudomonas fragi mutant strain, suitable for protein sequencing, lyophilized powder

grade

Proteomics Grade

Quality Segment

200

form

lyophilized powder

analyte chemical class(es)

amino acids

packaging

vial of 2 μg

suitability

suitable for protein sequencing

storage temp.

2-8°C

Description

General description

Endoproteinase Asp-N is a metallo endoprotease. It is obtained from a mutant strain of Pseudomonas fragi, which hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues.[1] Asp-N is used in proteomics for peptide mapping and protein sequence work due to its highly specific cleavage of peptides.

Application

Endoproteinase Asp-N from Pseudomonas fragi mutant strain has been used for the digestion of specific proteins to prepare peptides and for the analysis of generated peptides by MS (mass spectrometry) method.

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This Item	324708	P6181	324715
Sigma-Aldrich P3303 Endoproteinase Asp-N from Pseudomonas fragi mutant strain	Sigma-Aldrich 324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi	Sigma-Aldrich P6181 Endoproteinase Glu-C from Staphylococcus aureus V8	Sigma-Aldrich 324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
grade Proteomics Grade	grade Proteomics Grade	grade Proteomics Grade	grade Proteomics Grade
suitability suitable for protein sequencing	suitability -	suitability suitable for protein sequencing	suitability -
form lyophilized powder	form lyophilized solid	form lyophilized powder	form lyophilized
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 100
analyte chemical class(es) amino acids	analyte chemical class(es) -	analyte chemical class(es) amino acids	analyte chemical class(es) -