The product is tested in terms of potency per volume. There is no way to convert that into molar concentration unfortunately.
N2876
Neuraminidase from Clostridium perfringens (C. welchii)
Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder
Synonym(s):
Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-624-6
MDL number:
Specific activity:
≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA)
Pricing and availability is not currently available.
Quality Segment
type
Type V
form
lyophilized powder
specific activity
≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA)
application(s)
diagnostic assay manufacturing
foreign activity
Protease and NAN-aldolase, present
shipped in
dry ice
storage temp.
−20°C
Gene Information
Clostridium perfringens str. 13 ... nanI(988807)
General description
Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.
Application
Biochem/physiol Actions
Neuraminidase can increase aggregation in certain cell lines by removing exposed negatively charged sialic acid residues on the cell surface. [3]
Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.
Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.
The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.
Preparation Note
Prepared by salt fractionation.
Analysis Note
Package sizes based on the 4MU-NANA units
Package sizes based on 4MU-NANA units
Other Notes
One unit will liberate 1.0 micromole of N-acetyl neuraminic acid per minute at pH 5.0 at 37 °C using bovine submaxillary mucin.
One unit will hydrolyze 1.0 micromole of 2′-(4-methylumbelliferyl)-a-D-N-actetylneuraminic acid per minute at pH 5.0 at 37 °C (using 4MU-NANA as a substrate)
One unit will hydrolyze 1.0 micromole of 2′-(4-methylumbelliferyl)-a-D-N-actetylneuraminic acid per minute at pH 5.0 at 37 °C (using 4MU-NANA as a substrate)
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA) | specific activity ≥2 units/mg protein (mucin), ≥6 units/mg protein (using 4MU-NANA) | specific activity ≥50 units/mg protein (using 4MU-NANA) | specific activity 1-5 units/mg protein (Lowry, using NAN-lactose) |
| Gene Information Clostridium perfringens str. 13 ... nanI(988807) | Gene Information Clostridium perfringens str. 13 ... nanI(988807) | Gene Information Clostridium perfringens str. 13 ... nanI(988807) | Gene Information - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form buffered aqueous solution |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) diagnostic assay manufacturing |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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I noticed from previous answers, the strorage concentration should be 2mg/ml. But the package is 25U not 25mg, how can I convert U into mg? Or actally it's exact 1U for 1mg? Thanks!
1 answer-
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So it is not totally clear for me, if I order 25UNITS of this product, and I dissolve it 25 mL of 0.2% BSA solvent OR 25 mL of 10mM phosphate buffer (with 25-150 mM KCl, and without BSA) I will get 1U/ml enzyme solvent for my hemagglutination reaction?
1 answer-
Yes. Dissolving 25 units of an enzyme in 25 ml of various solvents results in a final concentration of 1 mg/ml of the enzyme.
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What should I use to dilute this product?
1 answer-
For assay purposes, this product is reconstituted at 0.02 - 0.04 u/mL in water containing 0.2% bovine serum albumin (BSA). The enzyme may also be prepared as follows:
Water containing 0.2% BSA at 2 mg/mL yielding a clear tan or brown solution
10 mM phosphate buffer, pH 6, containing 25 to 150 mM KCl at 120ug/mL. This stock solution may be stored refrigerated for several months.Helpful?
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Does this enzyme cleave Neu5Gc?
1 answer-
Product N2876, Neuraminidase, will cleave α2-3, α2-6, and α2-8 glycosidic linkages. Neu5Gc are mostly found terminally on glycan chains of glycoproteins and glycolipids. They are commonly linked via an α2-3 or α2-6 linkage to Gal, an α2-6 linkage to GalNAc, or via an α2-8 linkage to another sialic acid.
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What is the gene sequence for this product?
1 answer-
See the link below to review the gene information of Clostridium perfringens str. 13 nanI(988807) on NCBI:
https://www.ncbi.nlm.nih.gov/gene/988807The specific sequence of the orgnism use in the production of this product is considered proprietary.
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How should lyophilized neuraminidase be resuspended for storage in aqueous form? I plan to introduce it to cell culture.
1 answer-
The recommendation is to dissolve in water containing 0.2% BSA (albumin) at 2 mg/mL. This will yield a clear tan or brown solution. Neuraminidase is a comparatively stable enzyme in solution. When dissolved at 120 µg/mL in 10 mM phosphate buffer, pH 6, containing 25 to 150 mM KCl, the enzyme retained activity at 0-4 C for over 30 months. At a concentration of 2 µg/mL under the same conditions, activity was lost unless albumin (BSA) was added.
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