MTOX1001

MDR1 Knockout Caco-2 Cells

human male colorectal tissue (Source Disease: colon adenocarcinoma)

• Synonym(s): C2BBe1 Cells MDR1 (-/-/-/-)
• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: human male colorectal tissue (Source disease: colon adenocarcinoma)

Properties

• Product Name: MDR1 Knockout Caco-2 Cells, one vial
• biological source: human male colorectal tissue (Source disease: colon adenocarcinoma)
• Quality Segment: 200
• form: liquid
• technique(s): drug transporter assay: suitable, permeability assay: suitable
• OMIM accession no.: 171050
• application(s): ADME/TOX
• storage temp.: −196°C
• Gene Information: human ... ABCB1(5243)

Description

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 (MDR1) efflux transporter.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional knockout of the MDR1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MDR1 substrate
• The 24 well Transwell format enables the MDR1 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Legal Information

ADME/Tox Cell Lines License

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Also Available Separately

• MTOX1001

  MDR1 Knockout Caco-2 Cells, one vial 1 vial

  SDS

Safety Information

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 12 - Non Combustible Liquids