If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
MAK151
Fluorometric Thiol Assay Kit
sufficient for 200 fluorometric tests
Synonym(s):
Thiol Fluorescent Assay Kit
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About This Item
usage
sufficient for 200 fluorometric tests
detection method
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
1 of 1
This Item | |||
|---|---|---|---|
| usage sufficient for 200 fluorometric tests | usage sufficient for 100 fluorometric tests | usage sufficient for 200 fluorometric tests | usage sufficient for 200 fluorometric tests |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) endocrinological disorders, diabetes; cancer | relevant disease(s) cancer | relevant disease(s) endocrinological disorders, diabetes | relevant disease(s) neurological disorders |
Storage Class
10 - Combustible liquids
wgk
WGK 3
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Can this kit be used to quantify cysteines from proteins? If so, do I need to reduce disulfide bonds first? Also, does maleimide interfere with the assay?
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This kit detects reduced thiols such as reduced Glutathione (GSH) and Cysteine. In addition, the product may also be used to detect SH groups in the protein, provided that a standard with known SH groups is used, such as BSA (0.5-1 GH/molecule). The possible interference of Meleimide has not been investigated.
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Would MAK151 detect reduced thiols like GSH, Cysteine, and NAC, or does it detect total thiol? Additionally, does it detect total protein-SH or protein-S-S protein?
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MAK151 is capable of detecting reduced thiols like GSH, Cysteine, etc. It can also be used to detect SH groups in proteins, provided that known SH groups of the protein standard, such as BSA (0.5-1 GH/molecule), are available.
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