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M5775

Minimum Essential Medium Eagle

With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name

Minimum Essential Medium Eagle, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
Hanks’ salts (2% CO2): yes
L-glutamine: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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This Item
M4780M1018M4642
Minimum Essential Medium Eagle With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M5775

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Hanks′ salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M4780

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M1018

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M4642

Minimum Essential Medium Eagle

form

liquid

form

liquid

form

powder

form

powder

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
Hanks’ salts (2% CO2): yes
L-glutamine: no
sodium pyruvate: no

components

Earle’s salts (5% CO2): no
stable glutamine: no
Hanks’ salts (2% CO2): yes
phenol red: yes
NaHCO3: yes
sodium pyruvate: no
HEPES: no
glucose: yes
L-glutamine: yes

components

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

components

glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL8350
RNBL5428
RNBL1761
RNBK5418
RNBJ4240

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Maruša Bizjak et al.
Biochemical and biophysical research communications, 515(2), 296-302 (2019-05-31)
Metabolic pathways of cancer cells depend on the concentrations of nutrients in their micro-environment as well as on the cell-to-cell interactions. Here we examined the effects of glucose, pyruvate and glutamine on the sensitivity of MDA-MB-231 cells to metabolic drug metformin
Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
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Global Trade Item Number

SKUGTIN
M5775-6X500ML04061834060352
M5775-100ML04061834060338
M5775-500ML04061834060345

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