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Lipase from Mucor miehei

Name: Lipase from <I>Mucor miehei</I>
Brand: SIGMA

lyophilized powder, ≥4,000 units/mg solid (using olive oil)

Synonym(s):

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:

9001-62-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-619-9

EC Number:

3.1.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00131509

Specific activity:

≥4,000 units/mg solid (using olive oil)

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Properties

form

lyophilized powder

Quality Level

100

specific activity

≥4,000 units/mg solid (using olive oil)

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Lipase is produced by several animals, microbes, and plants. Lipase from Mucor sp. is a fungal lipase. It is a member of the serine hydrolase family.[1]

Application

Lipase from Mucor miehei can be used to drive the synthesis of flavor esters. [2]

Lipase from Mucor miehei has been used in a study to assess the nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. [3] It has also been used in a study to investigate the hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor. [4]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Microbial lipase has a wide industrial application due to its stability, broad substrate specificity, and selectivity. It may be used in the synthesis of biosurfactants and finds application in organic chemical processing, dairy industry, oleochemical industry, detergent formulations, paper, cosmetics, nutrition, and the pharmaceutical industry.[5]

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

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This Item	L8906	L8525	62310
Sigma-Aldrich L9031 Lipase from Mucor miehei	Sigma-Aldrich L8906 Lipase from Mucor javanicus	Sigma-Aldrich L8525 Lipase from Candida rugosa	Sigma-Aldrich 62310 Lipase from Rhizopus niveus
specific activity ≥4,000 units/mg solid (using olive oil)	specific activity ≥300 units/mg solid (using olive oil)	specific activity ≥40,000 units/mg protein	specific activity ≥1.5 U/mg
form lyophilized powder	form lyophilized powder	form lyophilized powder	form powder (fine)
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 100	Quality Level -	Quality Level 200	Quality Level 100