L8629

Lectin from Phaseolus vulgaris (red kidney bean)

Erythroagglutinin PHA-E

• Synonym(s): Phaseolus vulgaris agglutinin, PHA
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• Quality Segment: 300
• form: salt-free, lyophilized powder
• potency: <10 μg per mL agglutination activity (with erythryocytes)
• composition: Protein, ≥80% biuret
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Preparation Note

Purified by a modification of the method of Leavitt.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (10⁷ per mL in saline) after 1 hr incubation at 25 °C.

Tested for leucoagglutination.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)