I9516

2-Propanol

≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent

• Synonym(s): sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
• Linear Formula: (CH₃)₂CHOH
• CAS Number: 67-63-0
• Molecular Weight: 60.10
• NACRES: NA.52
• PubChem Substance ID: 24896158
• UNSPSC Code: 12352104
• EC Number: 200-661-7
• MDL number: MFCD00011674
• Beilstein/REAXYS Number: 635639
• Assay: ≥99.5%
• Grade: Molecular Biology
• Technique(s): DNA extraction: suitable, RNA extraction: suitable
• Bp: 82 °C (lit.)
• Vapor pressure: 33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

Properties

• Product Name: 2-Propanol, BioReagent, ≥99.5%, Molecular Biology
• grade: Molecular Biology
• Quality Level: 200
• vapor density: 2.1 (vs air)
• vapor pressure: 33 mmHg ( 20 °C), 44 mmHg ( 25 °C)
• product line: BioReagent
• assay: ≥99.5%
• form: liquid
• autoignition temp.: 750 °F
• expl. lim.: 2.0-12.7 %, 93 °C
• technique(s): DNA extraction: suitable, RNA extraction: suitable
• impurities: ≤0.2% water; water
• color: colorless
• refractive index: n20/D 1.377 (lit.)
• bp: 82 °C (lit.)
• mp: −89.5 °C (lit.)
• solubility: water: soluble (completely)
• density: 0.785 g/mL at 25 °C (lit.)
• suitability: suitable for precipitation of nucleic acids
• storage temp.: room temp
• SMILES string: CC(C)O
• InChI: 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
• InChI key: KFZMGEQAYNKOFK-UHFFFAOYSA-N

Description

General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

• DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.[1]
• Protein crystallization.[2][3][4]
• Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.[5][6]

Application

2-Propanol has been used:

• in DNA and RNA extraction[7][8][9][10]
• for determination of hydroxyproline levels(The Merck Index, 12th ed)
• for checking cell viability by MTT assay[11]
• in microarray hybridization[12]

Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

Safety Information

• pictograms: GHS02,GHS07
• signalword: Danger
• hcodes: H225,H319,H336
• pcodes: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• target_organs: Central nervous system
• Storage Class: 3 - Flammable liquids
• wgk: WGK 1
• flash_point_f: 53.6 °F - closed cup
• flash_point_c: 12.0 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter