HT5012

Formalin solution, neutral buffered, 10%

case of 24 × 60 mL, histological tissue fixative

• Synonym(s): 10% NBF, 10% Neutral Buffered Formalin, Formaldehyde, Methanal
• UNSPSC Code: 12352114
• PubChem Substance ID: 24895888
• NACRES: NA.47
• MDL number: MFCD00003274

Properties

• biological source: synthetic
• Quality Level: 200
• packaging: case of 24 × 60 mL
• concentration: 10% formalin (approx. 4% formaldehyde)
• color: colorless
• pH: 6.90-7.10
• density: 1.080 g/cm³
• application(s): hematology; histology
• storage temp.: room temp
• SMILES string: [O-]#[C+H2]
• InChI: 1S/CH2O/c1-2/h1H2
• InChI key: WSFSSNUMVMOOMR-UHFFFAOYSA-N

Description

General description

10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.

• It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
• It is used for routine histological staining, providing clear and detailed images of cellular structures.[1]

Popular general purpose histological fixative.

Biochem/physiol Actions

Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.

Features and Benefits

• 10% NBF is well-suited for large throughput laboratories.
• It requires a relatively short period of fixation.
• It can be used for the long-term storage of tissue.

Packaging

Case containers are polypropylene with white leak-proof lids and are partially prefilled with 10% NBF.

Physical form

Neutral buffered solution

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302 + H332,H317,H341,H350
• pcodes: P202 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1
• wgk: WGK 3
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: 185.0 °F
• flash_point_c: 85 °C