H7791

IL-17A human

HumanKine^®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture

• Synonym(s): CTLA-8, Interleukin-17A human, IL-17
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD01094096
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 30-35 kDa (glycosylated)
• Impurities: ≤1 EU/μg

Properties

• Product Name: Interleukin-17A human, IL-17A, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤2 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 30-35 kDa (glycosylated)
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: Q16552
• storage temp.: −20°C
• Gene Information: human ... IL17A(3605)

Description

Biochem/physiol Actions

IL-17, initially known as CTLA-8, is a T cell-expressed polypeptide that appears to be involved in the regulation of the hematopoietic system. IL-17 has been shown to induce IL-6, IL-8, and G-CSF production by fibroblasts; all of these cytokines have demonstrated effects on hematopoiesis.

IL-17A belongs to the IL-17 family and is produced by Th17 cells, a subset of T helper cells. Interleukin 17 (IL-17), initially known as CTLA-8, is a T cell expressed polypeptide that appears to be involved in the regulation of the hematopoietic system. IL-17 has been shown to induce IL-6, IL-8, and G-CSF production by fibroblasts; all of these cytokines have demonstrated effects on hematopoiesis. IL-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines, upregulates production of growth factors that promote angiogenesis, and enhances expression of adhesion molecules in fibroblasts.

Initially known as CTLA-8; is a T cell-expressed polypeptide that appears to be involved in the regulation of the hematopoietic system; has been shown to induce IL-6, IL-8, and G-CSF production by fibroblasts; all of these cytokines have demonstrated effects on hematopoiesis.

Physical form

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl pH 7.4+ 250 mM NaCl

Preparation Note

HumanKine Interleukin-17A (IL-17A), expressed in human HEK 293 cells, is a glycosylated homodimer with an apparent molecular mass of 30-35 kDa due to glycosylation. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent induction of IL-6 secretion from NDHF adult fibroblasts.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable