This product has not been validated for immunohistochemistry (IHC). However, numerous citations exist using this product for IHC.
G1544
Anti-GFP, N-terminal antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Green Fluorescent Protein
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
300
Pricing and availability is not currently available.
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.
Immunogen
synthetic peptide corresponding amino acids 3-17 of GFP.
Application
Anti-GFP, N-terminal antibody produced in rabbit has been used:
- in immunoblotting
- in the histochemistry and immunohistochemistry
- in the immunoprecipitation
Biochem/physiol Actions
Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.
Specific for GFP fusion proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
1 of 1
This Item | |||
|---|---|---|---|
| biological source rabbit | biological source mouse | biological source mouse | biological source rabbit |
| antibody form affinity isolated antibody | antibody form purified antibody | antibody form ascites fluid | antibody form IgG fraction of antiserum |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate unconjugated | conjugate - | conjugate unconjugated | conjugate unconjugated |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| technique(s) immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts | technique(s) immunoblotting: 1-2 μg/mL using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein., immunoprecipitation (IP): 5-10 μg using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein. | technique(s) dot blot: suitable, indirect ELISA: suitable, western blot: 1:2,000 using purified recombinant GFP preparation | technique(s) immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins, western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins |
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Datasheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G1544-100UG | 04061833628270 |
-
Can this antibody be used in IHC?
1 answer-
Helpful?
-