F4516

Anti-Human IgG2−FITC antibody, Mouse monoclonal

clone HP-6014, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Human IgG2
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: HP-6014, monoclonal
• Application: DIBA, PIFA
• Citations: 12

Properties

• biological source: mouse
• Quality Segment: 300
• conjugate: FITC conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: secondary antibodies
• clone: HP-6014, monoclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): dot immunobinding: 1:32, particle immunofluorescence: 1:16
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and residues inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain light chains paired with the VH and CH1 domains of the heavy chains.

Application

Monoclonal Anti-Human IgG2-FITC antibody produced in mouse has been used in immunohistochemistry.

Biochem/physiol Actions

The antibody is specific for human IgG2 and is non-reactive with other IgG subclasses. This clone has been singled out as the most widely applicable IgG2 specific monoclonal antibody by the IUIS/WHO study.

Physical form

Solution in 0.01 M phosphate buffered saline, pH8, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8ºC for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)