A3415

Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti Sheep Antibody, Anti Sheep Antibody - Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey, Anti Sheep Hrp
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162847
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA (d)
• Citations: 62

Properties

• biological source: donkey
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: sheep
• technique(s): direct ELISA: 1:10,000
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Peroxidase antibody reacts with all sheep immunoglobulins.

Immunogen

Purified sheep IgG

Application

Anti-Sheep IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

rElisas and CHEkiT Elisas were performed on serum from Sarda milking ewes using HRP-conjugated donkey anti-sheep IgG at1:1000. The antibody was incubated for 30 minutes at 37 degrees.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H315,H317,H319,H411
• pcodes: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable