This product is prepared by heat-shock fractionation which tends to yield a higher purity of Fraction V Albumin. The product is not tested for the presence of globulins or fatty acids on a lot-to-lot basis. However, historical information indicates the presence of fatty acids with low to no IgG detected. Alpha-globulin has been detected in the past.
A3294
Bovine Serum Albumin
heat shock fraction, protease free, pH 7, ≥98%
Bovine Serum Albumin
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
CAS Number:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
Impurities:
protease, none detected
Pricing and availability is not currently available.
biological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
impurities
protease, none detected
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected, VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Albumin is abundantly present in human plasma. Increased levels of albumin result in dehydration. Albumin turnover is usually found in children with iron deficiency anemia. It is a predictive indicator for liver disease.
Application
Bovine Serum Albumin has been used:
- to block non-specific binding of the antibodies
- as a component of activating medium for inducing sperm motility
- to determine the protein concentration of the bacteriocin by the bicinchoninic acid (BCA) method
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Heat shock fractionated
- Protease-free
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
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This Item | |||
|---|---|---|---|
| assay ≥98% | assay ≥96% (agarose gel electrophoresis) | assay ≥96% | assay ≥98% |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) ELISA: suitable, microbiological culture: suitable, blood typing: suitable | technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, blocking: suitable, electrophoresis: suitable, western blot: suitable | technique(s) immunocytochemistry: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Cryopreservation of Atlantic cod Gadus morhua L. spermatozoa: effects of extender composition and freezing rate on sperm motility, velocity and morphology
Butts I A E, et al.
Cryobiology, 61(2), 174-181 (2010)
Histomorphometric alteration and cell-type specific modulation of arylhydrocarbon receptor and estrogen receptor expression by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and 17beta-estradiol in mouse experimental model of endometriosis
Kitajima M, et al.
Reproductive Toxicology, 18(6), 793-801 (2004)
Leuconostoc carnosum 4010 has the potential for use as a protective culture for vacuum-packed meats: culture isolation, bacteriocin identification, and meat application experiments
Budde B B, et al.
International Journal of Food Microbiology, 83(2), 171-184 (2003)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A3294-10G | 04061835553297 |
| A3294-5G | 04061833425893 |
| A3294-100G | 04061835553280 |
| A3294-50G | 04061833360651 |
| A3294-1KG | 04061833360637 |
| A3294-500G | 04061833360644 |
| A3294-10KG | 04061833484029 |
| A3294-5KG | 04061833458334 |
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Does BSA 3294 Contain Fatty acid and globulin?
1 answer-
Helpful?
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こちらのBSAはシグナル配列(N末端からの18AA)が切断されているものと考えてよいでしょうか。
1 answer-
The signal sequence is not determined for Bovine Serum Albumin products. The signal/target sequence for BSA is most often associated with the preproalbumin in vivo. See the link below, section 3.1, for further information.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401331/Helpful?
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