This product is soluble in water at 20 µL per vial. The usage concentration is dependent on specific application details. An application discussion can be found on our website link:
https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-labeling-and-modification/ni-nta-atto-conjugates
94159
NTA - Atto 550
BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)
Synonym(s):
Atto 550 - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
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About This Item
NACRES:
NA.32
UNSPSC Code:
41116100
Pricing and availability is not currently available.
product line
BioReagent
Quality Level
assay
≥80% (sum of isomers, HPCE)
form
lumps
fluorescence
λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
Application
for the fluorescence detection of HIS-tagged proteins
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - | suitability suitable for fluorescence |
| fluorescence λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide | fluorescence λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide | fluorescence λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0 | fluorescence λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0 |
| form lumps | form solid | form solid | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| assay ≥80% (sum of isomers, HPCE) | assay ≥80% (sum of isomers, HPCE) | assay - | assay - |
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Eye Irrit. 2 - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 94159-250UG-F | 04061834412281 |
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What is the recommended stock solution concentration and usage concentration of NTA-ATTO 550?
1 answer-
Helpful?
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what is the molecular weight of NTA - Atto 550?
1 answer-
Unfortunately, the molecular weight of this product is not determined.
Helpful?
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What are the best conditions to store the reconstituted fluorophore? What would the shelf-life be under such conditions?
1 answer-
Upon receipt the material can be stored between 0 to -20 °C. After reconsitituion, the material can be
stored at 4 °C. The stability of the product has not been determined in reconstituted format.Helpful?
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