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71736

Sodium dodecyl sulfate solution

greener alternative

BioUltra, Molecular Biology, 10% in H2O

Synonym(s):

Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution

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About This Item

Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
NACRES:
NA.26
PubChem Substance ID:
eCl@ss:
39093306
UNSPSC Code:
12161902
MDL number:
Beilstein/REAXYS Number:
3599286
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grade

Molecular Biology

Quality Segment

description

anionic

product line

BioUltra

form

liquid

mol wt

288.38 g/mol

greener alternative product characteristics

Catalysis
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

10% in H2O

technique(s)

HPLC: suitable, protein quantification: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

pH

5.0-6.5

density

1.01 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg, phosphate (PO43-): ≤1 mg/kg

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.3, λ: 280 nm Amax: 0.2

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

application(s)

(Western Blot)

greener alternative category

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is used as a surfactant to enhance catalysis.  Click here for more information.

Application

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer
  • as a component in elution buffer for immunoprecipitation
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

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This Item
0503062862L5750
form

liquid

form

liquid

form

(Rods)

form

-

grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

-

technique(s)

HPLC: suitable, protein quantification: suitable

technique(s)

HPLC: suitable, protein quantification: suitable

technique(s)

LC/MS: suitable, electrophoresis: suitable, protein quantification: suitable

technique(s)

: suitable using [cell culture | mammalian: suitable]

application(s)

(Western Blot)

application(s)

(Pharmaceuticals, cosmetics)

application(s)

(Western Blot)

application(s)

(Western Blot)

mol wt

288.38 g/mol

mol wt

288.38 g/mol

mol wt

288.38 g/mol

mol wt

288.38 g/mol

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Ca: ≤5 mg/kg, Co: ≤1 mg/kg, Cu: ≤1 mg/kg, K: ≤20 mg/kg, Mg: ≤1 mg/kg, Mo: ≤1 mg/kg, Pb: ≤1 mg/kg, Zn: ≤1 mg/kg, As: ≤0.1 mg/kg, Cd: ≤1 mg/kg, Fe: ≤1 mg/kg, Mn: ≤1 mg/kg, Sr: ≤1 mg/kg, Bi: ≤1 mg/kg, Li: ≤1 mg/kg, Cr: ≤1 mg/kg, Ni: ≤1 mg/kg

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

-

cation traces

-


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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Certificates of Analysis (COA)

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Dictyostelium cultivation, transfection, microscopy and fractionation
Hirst J, et al.
Bio-protocol, 5(11) (2015)
Resilient Ribonucleases
RNA Methodologies (2010)
Construction of FWPV Chimaeric MVA
Buttigieg K R and Skinner M A
Bio-protocol, 5(1) (2015)



Global Trade Item Number

SKUGTIN
71736-100ML04061832851549
71736-500ML04061838254252

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. I WOULD LIKE TO REQUEST THE QUALITY CONFIRMATION OF %CONCENTRATION, SINCE CONCENTRATION IS 10% IN WATER, THERE'S NO INFORMATION THAT WHAT'S % TYPE [%W/V OR %V/V] REF.PRODUCT CODE: 71736

    1 answer
    1. This product is 10% (w/w) solution.

      Helpful?

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