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RPMI-1640 Medium

Name: RPMI-1640 Medium
Brand: SIGMA

with 2.05 mM L-glutamine, with 25mM HEPES, liquid, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217820

Pricing and availability is not currently available.

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Properties

description

for research or for further manufacturing use

Quality Segment

500

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

components

HEPES: 5958 mg/L
L-glutamine: 300 mg/L
NaHCO₃: 2000 mg/L
phenol red: 5.310 mg/L

shipped in

ambient

storage temp.

2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used to incubate dissected lung tissue.

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This Item	R7513	R1145	R1780
SAFC 51536C RPMI-1640 Medium	Sigma-Aldrich R7513 RPMI-1640 Medium	SAFC R1145 RPMI-1640 Medium	Sigma-Aldrich R1780 RPMI-1640 Medium
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form liquid
components HEPES: 5958 mg/L L-glutamine: 300 mg/L NaHCO₃: 2000 mg/L phenol red: 5.310 mg/L	components HEPES: no sodium pyruvate: no phenol red: yes L-glutamine: no NaHCO₃: yes	components NaHCO₃: no phenol red: yes sodium pyruvate: no HEPES: no L-glutamine: no	components NaHCO₃: 2 g/L phenol red: no glucose: 2 g/L (Dextro) L-glutamine: 0.3 g/L
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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