Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
12306C
Fetal Bovine Serum
non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture
Fetal Bovine Serum
Synonym(s):
HI FBS, HI FCS, HI Fetal Bovine Serum, HI Fetal Calf Sera, Heat Inactivated Fetal Calf Sera, FBS, FCS, sera, serum
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About This Item
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered
Pricing and availability is not currently available.
biological source
bovine fetus
Quality Level
sterility
sterile-filtered
product line
FBS Premier
quality
from USDA approved countries
composition
Hemoglobin, ≤25 mg/dL
origin
non-USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
storage temp.
−20°C
Application
FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.
Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.
Biochem/physiol Actions
Fetal Bovine Serum (FBS) is used as a supplement to cell culture media.[1] It provides many non-defined growth promoting and survival enhancing factors to cells in culture.
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
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This Item | |||
|---|---|---|---|
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| origin non-USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
| composition Hemoglobin, ≤25 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤20 mg/dL |
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 12306C-50ML | 04061837031175 |
| 12306C-100ML | 04061838722003 |
| 12306C-500ML | 04061838722010 |
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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