MABC002

Mouse IgG1 Negative Control, clone Ci4

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

• UNSPSC Code: 12352203
• NACRES: NA.42
• eCl@ss: 32160702
• Clone: Ci4, monoclonal
• Technique(s): flow cytometry: suitable, immunofluorescence: suitable
• Application: FACS, IF
• Citations: 20

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• clone: Ci4, monoclonal
• manufacturer/tradename: Chemicon^®
• concentration: 0.5 mg/mL
• technique(s): flow cytometry: suitable, immunofluorescence: suitable
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Immunogen

Chicken immunoglobulin

Application

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

Mouse IgG1 is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.

Research Category
Secondary & Control Antibodies

Epitope Tags & General Use

Research Sub Category
Isotype Control Antibodies

Biochem/physiol Actions

Reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.

Physical form

Format: Purified

Protein A Purified mouse immunoglobulin in phosphate buffered saline, pH 7.4, containing 0.2% bovine Serum albumin and 0.1% sodium azide.

Protein A purified

Preparation Note

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable