MAB5412

Anti-Indoleamine 2,3-dioxygenase Antibody, clone 10.1

clone 10.1, Chemicon^®, from mouse

• Synonym(s): Indoleamine-pyrrole 2,3-dioxygenase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 10.1, monoclonal
• Application: IHC, WB
• Citations: 43

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 10.1, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG3
• NCBI accession no.: NM_002164.4
• UniProt accession no.: P14902
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IDO1(3620)

Description

General description

42-45kDa

Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

Immunogen

Amino acids 78-184 of human IDO fused to GST.

Application

Anti-Indoleamine 2, 3-dioxygenase Antibody, clone 10.1 is an antibody against Indoleamine 2 for use in IH & WB.

Immunohistochemistry:
A previous lot of this antibody worked on lightly fixed tissues; Triton X-100 treatment only in the block, not with primary antibody. High temperature antigen retrieval is recommended.

Western blot:
Recognizes a 42-45kDa band in IFN-gamma treated human HeLa cell lines. Mouse IDO runs slightly smaller in blots than human IDO.

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Biochem/physiol Actions

Reacts with Indolamine 2,3-dioxygenase (IDO). Note IDO is usually expressed in response to various stimuli. A good positive control is HeLa cells stimulated with IFN-gamma, {http://www.upstate.com/browse/productdetail.q.ProductID.e.05-840#}.

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG3 in buffer containing 0.02 M phosphate, 0.25 M NaCl, 0.1% NaN3, pH 7.6.

Preparation Note

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Analysis Note

Control
IFN-gamma stimulated human peripheral blood lymphocytes, HeLa lysates.

Routinely evaluated by Western Blot on untreated and IFNgamma treated HeLa lysates.

Western blot:
1:500 dilution of this lot detected IDO on 10 μg of untreated and IFN gamma treated HeLa lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB5968

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• Storage Class: 12 - Non Combustible Liquids