189730

Streptavidin

• Synonym(s): Streptavidin, Avidin, Streptomyces avidinii
• CAS Number: 9013-20-1
• UNSPSC Code: 41116133
• NACRES: NA.41
• MDL number: MFCD00082035
• Form: lyophilized solid
• Biological source: (Streptomyces avidinii)

Properties

• biological source: (Streptomyces avidinii)
• Quality Level: 100
• form: lyophilized solid
• specific activity: ≥10 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A high affinity biotin binding protein (K_d approximately 10^-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.

A high-affinity biotin binding protein.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.

Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.

Other Notes

Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.

One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable