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TP0200

Total Protein Kit, Micro-Lowry, Onishi & Barr Modification

sufficient for ~50 manual assays

Synonyme(s) :

Total Protein Assay Kit

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A propos de cet article

NACRES:
NA.32
UNSPSC Code:
12352202
Service technique
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description

wavelength 500-750nm

Quality Segment

200

usage

sufficient for ~50 manual assays

protein detection level measuring range

0-1 mg/mL

parameter

18-26 °C temperature, 200 μL sample volume

storage temp.

2-8°C

General description

The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.

Application

Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
  • in the dialyzates/extracts of gliadins
  • in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
  • in the stored samples of rat liver microsomal fractions

Biochem/physiol Actions

Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS & Tarif
  • B3934
    Biuret reagent, protein detection level 150-1,000 μg/mL 110 mL
    FDS
  • Folin & Ciocalteu’s phenol reagent 5 mL
    FDS

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

Classe de stockage

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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