P9318

Monoclonal Anti-Plectin antibody produced in mouse

clone 7A8, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00164718
• Conjugate: unconjugated
• Clone: 7A8, monoclonal
• Application: ARR, DB, ELISA (i), ICC, IF, IHC (f), WB
• Citations: 9

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 7A8, monoclonal
• mol wt: antigen 300 kDa
• contains: 15 mM sodium azide
• species reactivity: marsupial (Ptoorous tridactylis PtK₂ cell line), rat
• technique(s): dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:200 using unfixed frozen sections of rat heart, microarray: suitable, western blot: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Plec1(64204)

Description

General description

Monoclonal Anti-Plectin (mouse IgG1 isotype) is derived from the 7A8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Plectin is an abundant, high molecular weight, cytomatrix protein (300 kDa) found in a wide variety of tissue and cell types. Plectin is found in stratified and non-stratified epithelia, fibroblasts, endothelial cells and astrocytes, as well as in striated, smooth and cardiac muscle, but not in neurons.

Immunogen

plectin from rat glioma C6 cells

Application

Monoclonal Anti-Plectin antibody produced in mouse has been used in:

• ELISA
• immunoblot
• dot blot
• immunocytochemistry
• immunoelectron microscopy

Biochem/physiol Actions

Plectin plays a role in the cross-linking of intermediate filaments, the interlinking of intermediate filaments with microtubules and microfilaments and the anchoring of intermediate filaments to the plasma membrane and the nuclear membrane. It is a part of the signal transduction mechanism involving kinases A and C, because in vitro as well as in vivo phosphorylation of the protein by those kinases differentially affected its binding affinities to vimentin and lamin B.

The antibody recognizes an epitope located in the middle section of the rod domain of the plectin molecule, approximately halfway between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as this antibody epitope. The antibody specifically labels the 300 kDa band of plectin, however, lower M.W. band(s) may also be detected. The antibody stains filamentous structures on immunohistochemical staining of frozen tissue sections. The antibody inhibits the interaction of plectin with vimentin and lamin B. An additional application for this antibody is immunoelectronmicroscropy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable