The minimum activity of this enzyme is 20 units per milligram. A survey of recent lots indicates a typical range of 45 - 50 units per milligram. This information is lot-specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l9795#product-documentation
L9795
Lactate Oxidase from Aerococcus viridans
lyophilized powder
Synonym(s):
L-lactate monooxygenase, lactate monooxygenase, lactate oxidative decarboxylase, lactate oxygenase, lactic oxidase, lactic oxygenase
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Biological source:
bacterial (Aerococcus viridans)
Pricing and availability is not currently available.
Quality Segment
biological source
bacterial (Aerococcus viridans)
form
lyophilized powder
mol wt
80 kDa (gel filtration)
storage temp.
−20°C
General description
Lactate oxidase (LOX) is a flavin mononucleotide (FMN)-dependent enzyme and is a member of the α-hydroxy-acid oxidase flavoenzyme family.
Application
Lactate Oxidase from Aerococcus viridans has been used as a:
- supplement in the culture medium to convert extracellular lactate to pyruvate
- coating on glass beads for electrochemical detection of lactate from a single cell by a push-pull nozzle system
- coating on multi-branched gold nanoparticles (GNPs) for the simultaneous colorimetric detection of lactate in saliva by paper-based multiplexed device
Biochem/physiol Actions
Lactate oxidase (LOX) catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide in the presence of an FMN co-factor. The enzyme aids in the determination of L-lactate indirectly by measuring the hydrogen peroxide formed in the reaction.
Other Notes
One unit is defined as the amount of enzyme which generates 1m mole of Hydrogen Peroxide per minute at 37°C under standard assay conditions.
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This Item | |||
|---|---|---|---|
| biological source bacterial (Aerococcus viridans) | biological source - | biological source - | biological source bacterial (Pediococcus spp.) |
| form lyophilized powder | form lyophilized powder | form powder | form lyophilized powder |
| mol wt 80 kDa (gel filtration) | mol wt - | mol wt - | mol wt ~76 kDa (gel filtration) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| L9795-500UN | 04061832730820 |
| L9795-100UN | 04061832730813 |
| L9795-50UN | 04061832730837 |
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What is the activity of this enzyme (U/g)?
1 answer-
Helpful?
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Hello, I am a research chemist. I would kindly like to know the excipients present in Lactate Oxidase from Aerococcus viridans L9795. Thank you in advance.
1 answer-
This freeze-dried preparation contains the following components:
Recombinant Lactate Oxidase at 15-20%
Sucrose at 50-84%
Potassium Phosphate monobasic at 1-30%Helpful?
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