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G4142

β-Galactosidase from bovine testes

ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)

Synonym(s):

β-D-Galactoside galactohydrolase

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About This Item

CAS Number:
9031-11-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
MFCD00130623
EC Number:
3.2.1.23 (BRENDA, IUBMB)
Specific activity:
1.0-3.0 units/mg protein (modified Warburg-Christian)
Biological source:
bovine
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biological source

bovine

Quality Level

200

form

ammonium sulfate suspension

specific activity

1.0-3.0 units/mg protein (modified Warburg-Christian)

foreign activity

α- and β-glucosidase ≤0.2%, α-fucosidase ≤0.2%, α-galactosidase ≤0.2%, α-mannosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%

storage temp.

2-8°C

Application

β-Galactosidase from bovine testes was used for coupling to Sepharose.[1]

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop[2].

Physical form

Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.

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This Item
G1875G4155G5635
β-Galactosidase from bovine testes ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)

Sigma-Aldrich

G4142

β-Galactosidase from bovine testes

β-Galactosidase from bovine liver Grade III, lyophilized powder, ≥0.15 units/mg protein

Sigma-Aldrich

G1875

β-Galactosidase from bovine liver

β-Galactosidase from Escherichia coli aqueous glycerol suspension, ≥500 units/mg protein (biuret)

Sigma-Aldrich

G4155

β-Galactosidase from Escherichia coli

β-Galactosidase from Escherichia coli Grade VIII, lyophilized powder, ≥500 units/mg protein

Sigma-Aldrich

G5635

β-Galactosidase from Escherichia coli

specific activity

1.0-3.0 units/mg protein (modified Warburg-Christian)

specific activity

≥0.15 units/mg protein

specific activity

≥500 units/mg protein (biuret)

specific activity

≥500 units/mg protein

biological source

bovine

biological source

bovine liver

biological source

-

biological source

-

form

ammonium sulfate suspension

form

lyophilized powder

form

aqueous glycerol suspension

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

foreign activity

α- and β-glucosidase ≤0.2%, α-galactosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%, α-fucosidase ≤0.2%, α-mannosidase ≤0.2%

foreign activity

-

foreign activity

-

foreign activity

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000500178
0000500178
0000500176
0000500176
0000375706

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Related Content

W Kiess et al.
The Journal of biological chemistry, 263(19), 9339-9344 (1988-07-05)
Cloning and sequencing of the human type II insulin-like growth factor (IGF) receptor cDNA revealed an 80% deduced amino acid sequence homology with the bovine cation-independent mannose 6-phosphate (Man-6-P) receptor, suggesting identity of the two receptors (Morgan, D. O., Edman
The purification and properties of beta-galactosidase from bovine testes.
J J Distler et al.
The Journal of biological chemistry, 248(19), 6772-6780 (1973-10-10)
D H Juers et al.
Protein science : a publication of the Protein Society, 8(1), 122-136 (1999-04-21)
Beta-galactosidase (lacZ) from Escherichia coli is a 464 kDa homotetramer. Each subunit consists of five domains, the third being an alpha/beta barrel that contains most of the active site residues. A comparison is made between each of the domains and
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Global Trade Item Number

SKUGTIN
G4142-.2UN04061832843766
G4142-.5UN04061832843773

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