Testing for bovine IgG and BSA is not conducted in the final product. A purity agarose gel is performed on F2379, but quantification, testing, or reporting for IgG and BSA is not done. Additionally, the marker size used for QC testing is uncertain, and both IgG and BSA are larger than fetuin.
F2379
Fetuin from fetal bovine serum
lyophilized powder, suitable for cell culture
Synonym(s):
Fetuin from fetal calf serum
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About This Item
Form:
lyophilized powder
Biological source:
bovine serum (fetal)
Pricing and availability is not currently available.
biological source
bovine serum (fetal)
Quality Level
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
impurities
≤0.3% free N-acetylneuraminic acid
solubility
H2O: 1 mg/mL, colorless to faint yellow or tan
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... FETUA(504615)
General description
Fetuin from fetal bovine serum is a 64 kDa plasma glycoprotein. It is produced by the liver and adipose tissue.
Application
Fetuin is a protein with the ability to bind to the insulin receptor and inhibit insulin action. Fetuin has been used in studies of type 2 diabetes mellitus (T2DM). In patients with T2DM, fetuin has been shown to be significantly lower when compared to control patients. Fetuin has also been used in studies to demonstrate its protective role in the development of arterial calcification.
Fetuin from fetal bovine serum has been used:
- to synthesize primary and secondary calciprotein particles (CPP)
- to study the binding of Xerocomus chrysenteron lectin (XCL) to the T antigen expressed by membrane proteins
- as a supplement in Ham′s F10 media for myoblast culture.
Biochem/physiol Actions
Fetuin, an inhibitor of insulin receptor tyrosine kinase, modulates bone remodeling and calcium metabolism. It is involved in keratinocytes migration and breast tumor cell proliferative signaling. Fetuin also regulates vascular calcification, insulin resistance and protease activity It acts as a biomarker for neurodegenerative diseases. Fetuin has anti-inflammatory property.
Preparation Note
Prepared by ammonium sulfate fractionation of fetal bovine serum by the method of Pederson, K.O., J. Phys. and Colloid Chem., 51, 164 (1947).
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This Item | |||
|---|---|---|---|
| biological source bovine serum (fetal) | biological source - | biological source bovine serum (fetal) | biological source bovine serum |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable, MALDI-MS: suitable | technique(s) cell culture | mammalian: suitable |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized solid |
| impurities ≤0.3% free N-acetylneuraminic acid | impurities ≤0.3% free N-acetylneuraminic acid | impurities ~0.2% free N-acetylneuraminic acid | impurities - |
| solubility H2O: 1 mg/mL, colorless to faint yellow or tan | solubility H2O: 1 mg/mL | solubility - | solubility water: 50 mg/mL |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Product Information Sheet
Cellular clearance and biological activity of calciprotein particles depend on their maturation state and crystallinity
Koeppert S, et al.
Frontiers in Immunology, 9, 1991-1991 (2018)
Kevan L Hartshorn et al.
American journal of physiology. Lung cellular and molecular physiology, 298(1), L79-L88 (2009-10-20)
Collectins are collagenous lectins present in blood, respiratory lining fluid, and other mucosal secretions that play important roles in innate defense against infection. The collectin, surfactant protein D (SP-D), limits infection by viruses and bacteria in the respiratory tract, eye
Pietro Allevi et al.
Journal of chromatography. A, 1212(1-2), 98-105 (2008-10-28)
The present report describes a method for the quantification of N-acetyl- and N-glycolylneuraminic acids without any derivatization, using their (13)C(3)-isotopologues as internal standards and a C(18) reversed-phase column modified by decylboronic acid which allows for the first time a complete
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F2379-250MG | 04061833261651 |
| F2379-5G | 04061833620229 |
| F2379-25G | 04061833629857 |
| F2379-1G | 04061833612910 |
| F2379-100MG | 04061833261644 |
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Is there information available on the content/concentration of bovine IgG and BSA in the fetuin products F2379 and F3385 (CAS 9014-81-7)?
1 answer-
Helpful?
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How pure is this fetuin? There's a protein content % but could there be other proteins from serum present when using ammonium sulfate fractionation?
1 answer-
Product F2379 can not be considered pure Fetuin. Neither the purity, nor the identity of potential contaminating proteins, is determined.
Helpful?
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Is there a good antibody to recognize this bovine fetuin B by western blot?
1 answer-
Unfortunately, the Fetuin antibodies currently available are not tested against bovine. While fetuin is fairly conserved from bovine to human, reactivity cannot be predicted. See the links below for fetuin antibodies against human that have been testing for use in western blot.
ABN1469
https://www.sigmaaldrich.com/product/mm/abn1469SAB5701234
https://www.sigmaaldrich.com/product/sigma/sab5701234Helpful?
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