This product is not anhydrous. The maximum allowable water content is < 0.1%. Please see the link below to review an anhydrous product option.
276855
https://www.sigmaaldrich.com/product/sial/276855
D8418
Dimethyl sulfoxide
Molecular Biology
Dimethyl sulfoxide
Synonym(s):
Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|
About This Item
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.52
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.9%
Grade:
Molecular Biology
Technique(s):
DNA sequencing: suitable, PCR: suitable, transfection: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
Pricing and availability is not currently available.
grade
Molecular Biology
Quality Level
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
product line
BioReagent
assay
≥99.9%
form
liquid
autoignition temp.
573 °F
shelf life
Recommended retest period - 2 years
expl. lim.
42 %, 63 °F
technique(s)
DNA sequencing: suitable, PCR: suitable, transfection: suitable
impurities
≤0.001 meq/g Titratable acid, ≤0.1% water (Karl Fischer)
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
absorption
passes test
suitability
suitable for molecular biology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
General description
Features and Benefits:
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
- Suitable for Molecular Applications
- Nuclease-Free DMSO
- DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
- Molecular Biology Grade DMSO
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
Application
DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in
Dimethyl sulfoxide has been used-
- polymerase chain reaction (PCR)
- amplification of cDNA libraries
- DNA sequencing
- column-loading buffers for poly(A)+ RNA selection
- buffers for the transformation of competent E. coli
- transfection protocols.
Dimethyl sulfoxide has been used-
- as an oligonucleotide solvent in array spotting[1]
- as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
- for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
- for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
- in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
Features and Benefits
This product is free from DNases, RNases, phosphatases and proteases.
Disclaimer
Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 400 | Quality Level - | Quality Level 300 |
| assay ≥99.9% | assay - | assay ≥99.9% (GC) | assay ≥99.9% (GC) |
| grade Molecular Biology | grade BioPerformance Certified | grade ACS reagent, puriss. p.a. | grade ACS reagent, puriss. p.a. |
| technique(s) DNA sequencing: suitable, transfection: suitable, PCR: suitable | technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) GC/GC: suitable | technique(s) GC/GC: suitable |
| solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility - | solubility - |
| form liquid | form liquid | form liquid | form liquid |
Still not finding the right product?
Explore all of our products under Dimethyl sulfoxide
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Product Information Sheet
Phillip McDonagh et al.
Veterinary microbiology, 174(3-4), 438-447 (2014-12-04)
Feline infectious peritonitis (FIP), a feline coronavirus (FCoV) induced disease, is almost invariably fatal with median life expectancy measured in days. Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen
A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8418-1L | 04061837896873 |
| D8418-1VL | 04061837299919 |
| D8418-5.5ML | 04061837299926 |
| D8418-500ML | 04054839596322 |
| D8418-1.5ML | 04061837299896 |
| D8418-1ML | 04061837299902 |
| D8418-100ML | 04061837896866 |
| D8418-7.5ML | 04061837299933 |
| D8418-50ML | 04061837896880 |
| D8418-250ML | 04061833589618 |
-
Is this product anhydrous?
1 answer-
Helpful?
-
-
May I know the concentration (mg/ml) for DMSO (cat no. D8418)?
1 answer-
This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.
Helpful?
-
-
Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?
1 answer-
Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:
-Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
-Moderately compatible: polystyrene, ECTFE/ETFE
-Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonatehttps://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf
Helpful?
-
-
Is this product sterile?
1 answer-
This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
https://www.sigmaaldrich.com/product/sigma/d2650
https://www.sigmaaldrich.com/product/sigma/d2438Helpful?
-
-
I received Product D8418, Dimethyl sulfoxide, as a frozen solid. But isn't it supposed to be in the liquid state?
1 answer-
DMSO super cools easily and remelts slowly at room temperature. The product may arrive as a solid instead of a liquid. The solidified product can be reliquified by warming to room temperature without detriment to the product.
Helpful?
-
-
What is the DMSO dissolved in?
1 answer-
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
Helpful?
-
-
Is Product D8418, Dimethyl sulfoxide, stable under extreme conditions?
1 answer-
DMSO is stable up to 100°C in alkaline, acidic and neutral conditions. At temperatures approaching its boiling point, DMSO is stable in neutral or alkaline conditions.
Helpful?
-
-
How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
-
-
Does DMSO have any synthetic utility?
1 answer-
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.
Helpful?
-
-
Dimethyl sulfoxide (DMSO) should be a liquid. Why did it arrive as a solid?
1 answer-
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
Helpful?
-