D8406

Anti -Dinitrophenyl antibody, Mouse monoclonal

IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Dinitrophenyl, Anti Dnp Ige, Anti-DNP IGE - Monoclonal Anti-Dinitrophenyl antibody produced in mouse, Anti-Dnp Ige, Monoclonal Anti-DNP
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: SPE-7, monoclonal
• Application: ELISA (i)
• Citations: 116

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: SPE-7, monoclonal
• form: buffered aqueous solution
• concentration: ~1 mg/mL
• technique(s): indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.
• isotype: IgE
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Hence, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP antibody is specific for DNP.
IgE gene is encoded on human chromosome 14. IgE is the least circulating antibody, but critical for eliciting hypersensitivity reaction against allergens.

Immunogen

DNP-KLH conjugate

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Dinitrophenyl antibody is suitable for use in flow cytometry and immunocytochemistry.
Monoclonal antidinitrophenyl specific mouse IgE has been used in degranulation of rat mast cells using microplate reader and fluorescence spectrophotometer.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Used to induce controlled allergic reactions or as a source of mouse IgE.

Biochem/physiol Actions

IgE is produced by B cells by class switch recombination in response to allergens. IgE is also associated with mast cells and is abundantly produced in response to allergens especially in asthma and allergic rhinitis. IgE regulates mast cell homeostasis and is upregulated in food allergy. IgE is implicated in the pathogenesis of autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus. IgE levels are modulated by other genes present on chromosome 14 especially in patients with asthma. Patients with mutations in signal transducer and activator of transcription 3 (STAT3) are associated high levels of IgE called the hyper–immunoglobulin E syndrome (HIES).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable