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A9469

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
alkaline phosphatase conjugate
Clone:
M2, monoclonal
Application:
ELISA (i)
Citations:
54
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biological source

mouse

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ALPL(249)

General description

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides).

Application

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used:
  • in direct tissue blot immunoassay of sweet orange petioles samples[1]
  • in screening internalization of delta opioid receptor
  • for screening cell-free protein expression using ELISA

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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F1804F3165F4049
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A9469

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse

ANTI-FLAG® M2 antibody, Mouse monoclonal 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

ANTI-FLAG® M2 antibody, Mouse monoclonal clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sigma-Aldrich

F3165

ANTI-FLAG® M2 antibody, Mouse monoclonal

ANTI-FLAG® M2-FITC antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F4049

ANTI-FLAG® M2-FITC antibody, Mouse monoclonal

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

form

buffered aqueous glycerol solution

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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