The control for this product is Product No. CL4B200 (Sepharose CL-4B).
C9210
Cyanogen bromide-activated Agarose
lyophilized powder
Synonyme(s) :
Agarose, cyclic carbonimidate, CNBr-Activated Agarose
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lyophilized powder
Quality Level
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix active group
cyanate or related structures
matrix spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling
1 g swells to 2.5-4.5 mL
capacity
≥10 mg/mL binding capacity (BSA)(via amino groups)
suitability
suitable for chromatography
storage temp.
−20°C
Application
Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.
Physical form
Lyophilized powder stabilized with lactose
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Cet article | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) - | technique(s) affinity chromatography: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form saline suspension |
| suitability suitable for chromatography | suitability suitable for chromatography | suitability - | suitability suitable for chromatography |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| matrix cross-linked 4% beaded agarose | matrix cross-linked 4% beaded agarose | matrix cross-linked 4% beaded agarose | matrix 4% beaded agarose |
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenu apparenté
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| C9210-15G | 04061833523278 |
| C9210-5G | 04061833523582 |
| C9210-1G | 04061833523285 |
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What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?
1 answer-
The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.
Helpful?
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What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?
1 answer-
Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare. In addition, C9210 is a cross-linked resin and C9142 is not.
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