There is no singular molecular weight for this product. Bile extract porcine is the dried fraction from hog bile, which is soluble in 80% alcohol. As estimated by TLC, this product contains Hyodeoxycholic acid at 1-5%, Deoxycholic acid at 0.5-7%, Cholic acid at 0.5-2%, Glycodeoxycholic acid at 10-15%, and Taurodeoxycholic acid at 3-9%.
B8631
Bile extract porcine
Synonyme(s) :
Bile acids, Hog Bile Powder
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A propos de cet article
Form:
powder
Biological source:
Porcine
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Service technique
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Laissez-nous vous aiderApplication
Bile extract porcine has been used:
- as a supplement in brain heart infusion (BHI) medium to culture Listeria monocytogenes LO28[1]
- to investigate the survival of probiotic bacteria through the gastric-small intestine[2]
- as a supplement in BHI mediato study the effect of oxygen on bile resistance in Listeria monocytogenes[3]
Chemical derivatization and HPLC have been proven for separation and identification of complex mixtures of bile acids and bile acid conjugates.
Biochem/physiol Actions
Bile from bovine and ovine can be used in bacteriological research to investigate bile salt adaptation of bacteria such as Lactobacillus.
Analysis Note
Contains glycine and taurine conjugates of hyodeoxycholic acid and other bile salts.
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Cet article | |||
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source Porcine | biological source bovine | biological source Porcine pancreas | biological source Porcine pancreas |
| form powder | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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mM 단위로 녹여서 사용하고자 하는데, 해당 제품의 분자량을 알수 있을까요?? 제품/MSDS 등에도 써있지 않아서 문의 남깁니다.
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What is the preparation method for B8631 and its solubility?
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The product B8631 should be considered a crude extract. In terms of solubility, quality control testing indicates that this product is tested for solubility in 80% ethanol at a concentration of 10 mg/mL, resulting in a slightly hazy to very hazy, very dark yellow solution. The color of the solution is within the acceptable range specified in the current Specification Sheet.
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I would like to know if this product contains taurocholic acid and sodium taurodeoxycholate and how much of each. Thank you
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Taurocholic acid and sodium taurodeoxycholate are not tested on a lot to lot basis for this product. However, historical data reveals that typically this material contains approximately 4-7% taurodeoxycholic acid and 30-40% taurocholic acid.
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What are the best solvents that can be used to solubilize this product?
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This product is soluble in 80% ethanol at 10mg/mL which yields a slightly hazy to very hazy solution. This material may also be dissolved in water at 50mg/mL. However, this will yield a turbid solution.
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What is the content of cholesterol (it should mostly be free cholesterol) in B8381 and B8631?
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Neither B8381 nor B8631 have been evaluated for cholesterol content. The approximate amount of known components of both are listed below:
8.0 - 9.5 % cholic acid
0.9 - 1.1 % deoxycholic acid
32 - 36 % conjugated cholic acid
11 - 13 % conjugated deoxycholic acidHelpful?
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What is the proper temperature for storage?
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This product is stored at room temperature.
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