Skip to Content
Merck

Key Documents

View All Documentation

Skip To

M4642

Minimum Essential Medium Eagle

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Minimum Essential Medium Eagle, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

Application

Minimum Essential Medium Eagle (MEM) has been used:

  • as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
  • as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures[1]
  • to culture Müller cells from guinea pigs

Preparation Note

Formulated to contain 10.6 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
M1018M0644M8042
Minimum Essential Medium Eagle With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M4642

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M1018

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M0644

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M8042

Minimum Essential Medium Eagle

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

powder

form

liquid

components

glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

components

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

MEM, classic media, classical media, FBS

MEM, classic media, classical media, FBS

classic media, classical media, FBS, hybrid cells

classic media, classical media, MEM-alpha, classic media, classical media, FBS

Still not finding the right product?

Explore all of our products under Minimum Essential Medium Eagle

Storage Class

11 - Combustible Solids

wgk

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000407703
0000407704
0000407703
0000407704
0000407649

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium
Iwona M G, et al.
Journal of Neuroimmunology, 161, 49-60 (2005)
Mast cell anaphylatoxin receptor expression can enhance IgE-dependent skin inflammation in mice
Beatrix S, et al.
The Journal of Allergy and Clinical Immunology, 131(2), 541-548 (2013)
Anthony Dudilot et al.
Current biology : CB, 30(7), 1167-1176 (2020-02-23)
Synaptic plasticity, with its two most studied forms, long-term potentiation (LTP) and long-term depression (LTD), is the cellular mechanism underlying learning and memory. Although it has been known for two decades that bidirectional synaptic plasticity necessitates a corresponding bidirectional regulation
View All Related Papers

Global Trade Item Number

SKUGTIN
M4642-50K04061834171935
M4642-10L04061834059097
M4642-50L04061834059103
M4642-BIBC04061826573891

Questions

Reviews

No rating value

Active Filters