E2761

ExtrAvidin^®−FITC

buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00163600
• Form: buffered aqueous solution

Properties

• conjugate: FITC conjugate
• Quality Segment: 200
• form: buffered aqueous solution
• extent of labeling: 3-6 mol fluorochrome per mol protein
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[1] It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[2] ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.

Application

ExtrAvidin^®-FITC is suitable for the following applications:

• immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.[3][4]
• Immunofluorescence[5][6]†
• competitive binding assay[7]

Physical form

Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2