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B4750

Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride

trypsin substrate

Synonym(s):

BANA, Nα-Benzoyl-DL-arginine-2-naphthylamide hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C23H25N5O2 · HCl
CAS Number:
913-04-2
Molecular Weight:
439.94
UNSPSC Code:
12352204
NACRES:
NA.32
PubChem Substance ID:
24891699
EC Number:
213-015-4
Beilstein/REAXYS Number:
3823291
MDL number:
MFCD00038940
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Quality Segment

200

assay

≥98% (TLC)

form

powder

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc3ccccc3c2

InChI

1S/C23H25N5O2.ClH/c24-23(25)26-14-6-11-20(28-21(29)17-8-2-1-3-9-17)22(30)27-19-13-12-16-7-4-5-10-18(16)15-19;/h1-5,7-10,12-13,15,20H,6,11,14H2,(H,27,30)(H,28,29)(H4,24,25,26);1H

InChI key

BNGRKDJZQIGWQF-UHFFFAOYSA-N

General description

A chromogenic substrate for trypsin.

Application

Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride has been used as a substrate in the BANA test to evaluate the inhibitory activity of stefin.[1] It has also been used as a substrate for the determination of Ki values of inhibitors toward trypsin.

Biochem/physiol Actions

Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride (BANA) helps to determine the activities of cathepsin B and papain. BANA test is also useful in identifying the number of periodontal sites with periodontal pathogens. Presence of volatile sulfur compounds (VSCs), synthesized by oral bacteria in dental plaque or tongue coating can be colorimetrically confirmed by their ability to hydrolyze N-benzoyl-DL-arginine-2-naphthylamide.

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This Item
B4875A2628B3279
Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride trypsin substrate

Sigma-Aldrich

B4750

Nα-Benzoyl-DL-arginine β-naphthylamide hydrochloride

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride ≥98%

Sigma-Aldrich

B4875

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

L-Alanine β-naphthylamide protease substrate

Sigma-Aldrich

A2628

L-Alanine β-naphthylamide

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride ≥98% (HPLC)

Sigma-Aldrich

B3279

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

assay

≥98% (TLC)

assay

≥98%

assay

≥98% (TLC)

assay

≥98% (HPLC)

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

solubility

ethanol: 50 mg/mL, clear to slightly hazy

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM7919
BCCG8291
BCBV2810
BCBQ3752V
BCBJ7198V

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Related Content

Product Information Sheet - B4750

Product Information Sheet

Global Trade Item Number

SKUGTIN
B4750-5G04061832921341
B4750-1G04061833433447
B4750-500MG04061832921334

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