The IL-10 standard is prepared by diluting 5 uL with 728.3 uL of Assay Diluent A or Assay Diluent B. The formulations of both diluents are proprietary and may not be shared. Please see the link below for the sample Certificate of Analysis to review the protocol. Note the standard preparation instructions on page 2: https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0477_1013J0430.pdf
RAB0477
Mouse Tumor Necrosis Factor α ELISA Kit
for serum, plasma and cell culture supernatant
Synonym(s):
Cachectin, TNF-alpha, Tumor necrosis factor alpha
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About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Tnf(21926)
Input:
sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type cell culture supernatant(s)
sample type plasma
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice
Pricing and availability is not currently available.
species reactivity
mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type serum
sample type cell culture supernatant(s)
sample type plasma
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Tnf(21926)
General description
Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.
The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.
Immunogen
Recombinant Mouse TNF-α
Application
Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples.
Biochem/physiol Actions
Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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This Item | |||
|---|---|---|---|
| Gene Information mouse ... Tnf(21926) | Gene Information rat ... Tnf(24835) | Gene Information Porcine ... TNF(397086) | Gene Information bovine ... TNF(280943) |
| species reactivity mouse | species reactivity rat | species reactivity porcine | species reactivity bovine |
| input sample type serum | input sample type serum | input sample type serum | input sample type plasma |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <10% |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
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How to prepare lyophilized reagents from ELISA kits for IL10 elisa sigma RAB 0244-1KT and on standard dilution: 5microl + 728.3 microl BUT WHAT IS 728.3 microl
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