No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.
PPC2020
Protease and Phosphatase Inhibitor Cocktail
ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidases, L-isozymes of alkaline phosphatases, non-freezing solution
Synonym(s):
Protease and phosphatase inhibitor cocktail, inhibitor cocktail
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
Pricing and availability is not currently available.
Product Name
ReadyShield® Protease and Phosphatase Inhibitor Cocktail, non-freezing solution
Quality Level
form
ready-to-use solution
packaging
pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
solubility
water: soluble
storage temp.
−20°C
General description
Protease and Phosphatase Inhibitor Cocktail has been optimized and tested for mammalian cellular and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, acid proteases, aminopeptidases, L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases.
Protease and Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.
The cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® PPC2020 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail PPC1010
Protease and Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.
The cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® PPC2020 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail PPC1010
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- For broad ranging inhibition of both proteases and phosphatases.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
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| form ready-to-use solution | form ready-to-use solution | form DMSO solution | form DMSO solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging pkg of 1 ml, 5 ml × | packaging - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
Extracts, Lysate, Cell Extracts, Tissue Extracts, Plant Tissue Extract, Mammalian Tissue Extract, Bacterial lysate, Electrophoresis, Phosphatase, phosphotyrosine, phosphoserine | Extracts, Lysate, Cell Extracts, Tissue Extracts, Bacterial lysate, Electrophoresis, Phosphatase, phosphotyrosine, phosphoserine | - | - |
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| PPC2020-1ML | 04061842555192 |
| PPC2020-5ML | 04061842555208 |
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Do ReadyShield® inhibitor cocktails include EDTA?
1 answer-
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What is the recommended storage temperature for ReadyShield® products?
1 answer-
The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).
Helpful?
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Does PPC2020 contain EDTA?
1 answer-
PPC2020 does not contain EDTA
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