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Elastase from porcine pancreas

Name: Elastase from porcine pancreas
Brand: SIGMA

lyophilized powder, suitable for cell culture

Synonym(s):

Elastase from hog pancreas, Pancreatopeptidase E

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About This Item

CAS Number:

39445-21-1

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

254-453-6

MDL number:

MFCD00130998

EC Number:

3.4.21.36 (BRENDA, IUBMB)

Specific activity:

≥4 units/mg protein

Biological source:

Porcine

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Properties

biological source

Porcine

Quality Level

200

form

lyophilized powder

specific activity

≥4 units/mg protein

mol wt

25.9 kDa

packaging

pkg of 1 μmol

technique(s)

cell culture | mammalian: suitable

pH

suitability

suitable for

foreign activity

Trypsin ≤50 BAEE units/mg protein

shipped in

dry ice

storage temp.

−20°C

Description

General description

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Application

Elastase from porcine pancreas is used for the following applications:

Cell culture techniques[1]
ELISA techniques[2]
Used in assay for elastase inhibitory activity[3]

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Packaging

Package size based on protein content

Other Notes

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

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1 of 1

This Item	E0127	E0258	E1250
Sigma-Aldrich E7885 Elastase from porcine pancreas	Sigma-Aldrich E0127 Elastase from porcine pancreas	Sigma-Aldrich E0258 Elastase from porcine pancreas	Sigma-Aldrich E1250 Elastase from porcine pancreas
biological source Porcine	biological source -	biological source Porcine pancreas	biological source Porcine pancreas
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) -
specific activity ≥4 units/mg protein	specific activity ≥4.0 units/mg protein	specific activity ≥4.0 units/mg protein (biuret)	specific activity ≥4.0 units/mg protein
suitability suitable for	suitability -	suitability -	suitability -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form suspension
mol wt 25.9 kDa	mol wt 25.9 kDa	mol wt -	mol wt 25.9 kDa

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000443624

0000443625

0000428612

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Atrial natriuretic factor-induced egression of cyclic guanosine 3':5'-monophosphate in cultured vascular smooth muscle and endothelial cells.

P Hamet et al.

The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)

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Global Trade Item Number

SKU	GTIN
E7885-5MG	04061835568420
E7885-20MG	04061835568413
E7885-1MG	04061835568406

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