D5751

Blue Dextran

Void volume marker for gel filtration columns.

• Synonym(s): Dextran blue from Leuconostoc mesenteroides
• CAS Number: 87915-38-6
• UNSPSC Code: 12352200
• NACRES: NA.25
• MDL number: MFCD00130661

Properties

• biological source: non-animal source
• Quality Segment: 200
• form: powder
• quality: 200
• mol wt: 2,000,000
• technique(s): affinity chromatography: suitable
• suitability: suitable for GFC marker

Description

General description

Blue Dextran is a soluble derivative of dextran. Its structure includes triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran. Blue Dextran is generally used as a marker to determine void volume in gel filtration chromatography columns.

Application

Blue Dextran has been used for calibration of size exclusion chromatography (SEC) column. It has also been used in Dextran blue permeability assays to determine the integrity of co-cultures containing epithelial cells. Blue dextran may be used in affinity chromatography, gel filtration chromatography, protein chromatography, and molecular weight markers. Blue dextran is also suitable to study Alzheimer′s disease.

Preparation Note

Prepared from dextran with an average mol. wt. of 2,000,000.

Analysis Note

degree of substitution: 0.10 to 0.12 mmol Cibracon Blue per gram of dextran

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)