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A3678

Ammonium persulfate

Molecular Biology, suitable for electrophoresis, ≥98%

Synonym(s):

AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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About This Item

Linear Formula:
(NH4)2S2O8
CAS Number:
Molecular Weight:
228.20
PubChem Substance ID:
eCl@ss:
38030421
UNSPSC Code:
41105319
NACRES:
NB.22
EC Number:
231-786-5
MDL number:
Assay:
≥98%
Form:
powder
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grade

Molecular Biology

Quality Level

vapor density

7.9 (vs air)

assay

≥98%

form

powder

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl-): <10 ppm

cation traces

Fe: <10 ppm, heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected, Protease, none detected, RNAse, none detected

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Catalyst for acrylamide gel polymerization.
Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Analysis Note

Tested for use in acrylamide polymerization.

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This Item
248614A7460908932
assay

≥98%

assay

≥98.0%

assay

≥98.0%

assay

≥98.0%

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

form

powder

form

chunks or granules, powder or crystals

form

powder

form

powder

cation traces

Fe: <10 ppm, heavy metals (as Pb): <50 ppm

cation traces

Fe: ≤0.001%, Mn: ≤0.5 ppm, heavy metals (as Pb): ≤0.005%

cation traces

Al: ≤0.0005%, Ca: ≤0.001%, Cu: ≤0.0005%, Fe: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0001%, Na: ≤0.01%, Pb: ≤0.001%, Zn: ≤0.0005%

cation traces

-

anion traces

chloride (Cl-): <10 ppm

anion traces

Cl- and ClO4-: ≤0.001%

anion traces

chloride (Cl-): ≤0.001%

anion traces

-


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signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions.
Chiang MY et al.
Biomaterials, 34, 9754-9754 (2013)
Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.
Fancy DA and Kodadek T
Proceedings of the National Academy of Sciences of the USA, 96, 6020-6020 (1999)
Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.
Liao IC et al.
Advances in Functional Materials, 23, 5833-5833 (2013)



Global Trade Item Number

SKUGTIN
A3678-25G04061835515707
A3678-100G04061835521531

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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