85578

Spermidine trihydrochloride

BioXtra, ≥99.5% (AT)

• Synonym(s): N-(3-Aminopropyl)-1,4-butanediamine trihydrochloride
• Linear Formula: NH₂(CH₂)₃NH(CH₂)₄NH₂ · 3HCl
• CAS Number: 334-50-9
• Molecular Weight: 254.63
• UNSPSC Code: 12352116
• NACRES: NA.26
• PubChem Substance ID: 57653005
• EC Number: 206-379-0
• Beilstein/REAXYS Number: 3552356
• MDL number: MFCD00012918

Properties

• product line: BioXtra
• Quality Segment: 100
• assay: ≥99.0% (TLC), ≥99.5% (AT)
• form: powder
• impurities: insoluble matter, passes filter test
• pH: 3.0-6.0 (25 °C, 1 M in H₂O)
• mp: 257-259 °C (lit.)
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤10 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.2, λ: 280 nm A_max: 0.1
• SMILES string: Cl[H].Cl[H].Cl[H].NCCCCNCCCN
• InChI: 1S/C7H19N3.3ClH/c8-4-1-2-6-10-7-3-5-9;;;/h10H,1-9H2;3*1H
• InChI key: LCNBIHVSOPXFMR-UHFFFAOYSA-N

Description

General description

Spermidine trihydrochloride is an aliphatic polyamine. Its crystalline structure has been studied. It has been reported to preserve the nuclei and prevent proteolytic degradation of histones during the macronuclei isolation from Tetrahymena pyriformis.[1]

Application

Spermidine trihydrochloride is used for the following applications:

• DNA extraction and restriction endonuclease digestion[2]
• Nuclear transplantation[3]
• During the chemical analyses of the squid species Todaropsis eblanae[4]
• As one of the agents in intercalating drug.[5]
• Platelet aggregation[6]

It may be used in the synthesis of palladium complexes like [sperH₃]₂[PdCl₄]₃, [PdCl₂(sperH)]₂[PdCl₄], and [(PdCl₂)₃(sper)₂] by reacting with K₂PdCl₄ and PdCl₂.[7]

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T₄ polynucleotide kinase activity.

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Other Notes

Buffer component for chromosome isolation in bivariate flow cytogenetic analysis and sorting[8].

Disclaimer

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves