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NA2110

Sigma-Aldrich

GenElute™ Bacterial Genomic DNA Kits

sufficient for 70 purifications

Sinônimo(s):

Bacterial Genomic DNA, bacterial DNA prep, bacterial DNA purification kit, bacterial gDNA extraction kit, bacterial gDNA isolation kit, bacterial gDNA purification kit, bacterial genomic DNA extraction kit, bacterial genomic DNA isolation kit, bacterial genomic DNA purification kit, Gen Elute

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.55

uso

sufficient for 70 purifications

técnica(s)

DNA purification: suitable

temperatura de armazenamento

15-25°C

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Descrição geral

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Aplicação

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Características e benefícios

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required

Princípio

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Outras notas

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informações legais

GenElute is a trademark of Sigma-Aldrich Co. LLC

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • W0263Wash Solution 1SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

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Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Central nervous system, Respiratory system

Código de classe de armazenamento

3 - Flammable liquids

Ponto de fulgor (°F)

77.0 °F - closed cup

Ponto de fulgor (°C)

25 °C - closed cup

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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