G1N350

GenElute^™ Mammalian Genomic DNA Miniprep Kits

sufficient for 350 purifications

• Sinônimo(s): Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
• Código UNSPSC: 41105501
• NACRES: NA.55

Propriedades

• uso: sufficient for 350 purifications
• técnica(s): DNA purification: suitable
• temperatura de armazenamento: 15-25°C

Descrição

Descrição geral

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Aplicação

GenElute^™ Mammalian Genomic DNA Miniprep Kits has been used:

• to extract DNA from fin samples
• to extract the BSTUI restriction enzyme from the DNA
• to isolate DNA from the blood samples extracted from caudal fin
• to extract tissue and hair follicle DNA

The purified genomic DNA is ready for immediate use in downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Características e benefícios

• Expected yield: 25 μg from 2 x 10⁶ cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Princípio

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Outras notas

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informações legais

GenElute is a trademark of Sigma-Aldrich Co. LLC

Componentes do kit também disponíveis separadamente

• C2112 Column Preparation Solution
• P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
• R6148 RNase A solution

Informações de segurança

• Pictogramas: GHS08,GHS05,GHS07,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H290,H302,H315,H318,H334,H335,H410
• Declarações de precaução: P234 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable