In general, MEM typically contains 0.292 g/L L-Glutamine. A 200mM Glutamine solution is equivalent to 29.23 mg/mL. So it needs to be diluted around 100 times.
G7513
L-Glutamina
L-Glutamine solution
200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
Sinônimo(s):
Glavamin, Levoglutamide
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About This Item
Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352209
ID de substância PubChem:
NACRES:
NA.75
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
esterilidade
sterile-filtered
Nível de qualidade
300
400
linha de produto
BioXtra
Formulário
solution
concentração
200 mM
técnica(s)
cell culture | mammalian: suitable
Impurezas
endotoxin, tested
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
chave InChI
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Informações sobre genes
rat ... Ggt1(116568)
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Descrição geral
A L-glutamina é um aminoácido essencial que é um componente fundamental dos meios de cultura, funcionando como uma fonte de energia importante para células em cultura. A L-glutamina é bastante estável como pó seco e como solução congelada. Em meios líquidos ou soluções estoque, no entanto, a L-glutamina se degrada de modo relativamente rápido. O desempenho ideal da célula, em geral, requer suplementação dos meios com L-glutamina antes do uso.
Aplicação
A L-glutamina é um aminoácido essencial que é um componente fundamental dos meios de cultura, funcionando como uma fonte de energia importante para células em cultura. O desempenho ideal da célula, em geral, requer suplementação dos meios com L-glutamina antes do uso.
Nota de preparo
Preparada em água de grau para cultura celular.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Chromatograms
application for HPLCapplication for HPLC-
How much of Product G7513 should be used in cell culture media?
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
1 answer-
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
1 answer-
L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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