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Documentos Principais

M3817

SAFC

MegaCell RPMI-1640 Medium

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

esterilidade

sterile-filtered

Formulário

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

L-glutamine: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Descrição geral

MegaCell is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.

Aplicação

MegaCell RPMI-1640 Medium has been used:
  • as a component of the TSC medium for culturing trophoblast stem cells (TSC)
  • as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
  • as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs

Reconstituição

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Informações legais

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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