D4889
Deoxyribonucleic acid sodium salt from Escherichia coli strain B
Genomic, unsheared
Sinônimo(s):
DNA
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About This Item
Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52
Produtos recomendados
grau
for molecular biology
Nível de qualidade
forma
lyophilized powder
peso molecular
>45 kb
temperatura de armazenamento
2-8°C
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Categorias relacionadas
Descrição geral
Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA.
Especificidade
50% GC; Tm = 90.5 °C
Aplicação
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
- PCR reactions of genomic DNA of Gardnerella vaginalis
- nick translation and radiolabeling of dNTP
- generating standard curve for 16S rDNA quantification
- transfecting neutrophils
- testing the extraction methods
Definição da unidade
One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).
Nota de preparo
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Nota de análise
Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater.
Outras notas
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
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Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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