D4764
Deoxyribonucleic acid from calf thymus
Genomic, unsheared
Sinônimo(s):
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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About This Item
Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52
Produtos recomendados
grau
for molecular biology
forma
lyophilized powder
peso molecular
>20 kb
temperatura de armazenamento
2-8°C
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Descrição geral
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Especificidade
42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Aplicação
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Características e benefícios
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Nota de preparo
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
Reconstituição
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves
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