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S8015

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

grau

for molecular biology

forma

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

atividade externa

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temperatura de armazenamento

room temp

Descrição geral

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Aplicação

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Características e benefícios

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Componentes

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

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Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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