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S8140

Sigma-Aldrich

SSPE Buffer

for Northern and Southern blotting, powder blend

Sinônimo(s):

Saline-Sodium Phosphate-EDTA Buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

grau

for molecular biology

forma

powder blend

pH

7.2-7.6 (20 × in water, high purity, ambient)

solubilidade

water, high purity: 20 ×, clear, colorless

atividade externa

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temperatura de armazenamento

room temp

Categorias relacionadas

Descrição geral

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

Aplicação


  • Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).

  • Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

Características e benefícios

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Componentes

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures
Igor V. Kutyavin
Nucleic Acids Research, 28(2) (2000)
Microfluidic-based enzymatic on-chip labeling of miRNAs
Sonja Vorwek
New Biotechnology (2008)

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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