Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Sinônimo(s):

MESA Buffer

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

grau

for molecular biology

linha de produto

BioReagent

forma

powder

técnica(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubilidade

H2O: soluble, faintly yellow

atividade externa

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

temperatura de armazenamento

room temp

Categorias relacionadas

Descrição geral

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Aplicação

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Componentes

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Reconstituição

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 3

1 of 3

MOPS anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD018

MOPS

MOPS, Free Acid, Molecular Biology Grade CAS 1132-61-2 is a zwitterionic buffer useful in the pH range of 6.5-7.9. Has a pKa of 7.20 at 25°C.

Millipore

475922

MOPS, Free Acid, Molecular Biology Grade

MOPS anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD003

MOPS

Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Electrophoresis in agarose and acrylamide gels.
R C Ogden et al.
Methods in enzymology, 152, 61-87 (1987-01-01)
Exibir todos os documentos relacionados

Protocolos

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica